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Integrated Multiplex Immunofluorescence (mIF) Technology
The DNBSEQ-G400RS FluoXpert utilizes advanced multiplex immunofluorescence (mIF) technology, enabling the detection of multiple target proteins within the same tissue section while preserving essential spatial information. This method allows for the comprehensive analysis of complex cellular compositions, functional phenotypes, and intercellular interactions, making it particularly advantageous for studying tumor microenvironments.
Unlike traditional immunohistochemistry (IHC), which requires multiple sections to examine different proteins, mIF can target several proteins in a single section, minimizing sample waste and maintaining tissue integrity. The integrated imaging system features a sophisticated optical setup and a high-resolution stitching algorithm, providing detailed and accurate imaging throughout cycles of staining and elution. This facilitates repeated analysis with minimal tissue damage.
High-throughput Sequencing Integration
The DNBSEQ-G400RS FluoXpert uniquely combines high-throughput genetic sequencing with multiplex immunofluorescence (mIF) staining capabilities, providing a comprehensive platform for simultaneous molecular and morphological analysis. This integration allows researchers to switch between sequencing and staining modes seamlessly, enhancing the device's utility in translational medicine research.
The sequencer's architecture supports various read lengths and throughput options, catering to diverse research needs and scales. Moreover, this dual functionality enables more efficient use of samples and reagents, reduces the operational footprint by eliminating the need for multiple devices, and accelerates multi-omics research by providing both proteomic and genomic data from the same sample.
Experimental Results
DNBSEQ-G400RS FluoXpert comes with a multiplex immunofluorescence reagent set. It is compatible with both frozen tissue and FFPE samples. It is also compatible with commercially available antibodies without a complex conjugation process. The results generated by FluoXpert showed no significant difference from the gold standard, IHC. It can be used in different fields of proteomics research
No significance is observed between mIF and the gold standard IHC
Mouse brain-FFPE section
Mouse brain-frozen sample
Lung adenocarcinoma-
FFPE section
Tonsil FFPE section
PanCK
PanCK/Nucleus
CD8
CD8/Nucleus

CD3
CD3/Nucleus
CD68
CD68/Nucleus
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