DNBSEQ Technology Banner

Home

/

Technology

DNBSEQ™ Technology

Discover the Power of DNBSEQ™ Technology
A Revolution in DNA Sequencing

At MGI, we are pioneering a new era of genomic exploration through our innovative DNBSEQ™ technology. By merging cutting-edge engineering with a deep understanding of biology, we empower researchers, clinicians, and industry leaders worldwide to unlock genomic insights with unprecedented accuracy, efficiency, and value.

Reduced Index Hopping & Bias

Circularized templates and RCR minimize error accumulation, improving variant detection accuracy.

Optimized Throughput & Efficiency

Patterned Arrays and precise DNB loading increase signal density, enabling scalable, cost-effective sequencing.

Enhanced Base Calling Precision

Sub-pixel Registration and GPU-accelerated processing deliver faster, more reliable base calls.

High-Fidelity Sequencing

MGI’s DNBSEQ™ technology uses single-strand DNA circularization and rolling circle replication (RCR) to generate DNA Nanoballs (DNBs) directly from the original template.

This approach, combined with Patterned Arrays, cPAS chemistry, and advanced image analysis, reduces index hopping and biases, ensuring more accurate, high-quality sequencing data for both whole-genome and targeted applications.

How DNBSEQ™ Works

MGI’s DNBSEQ™ technology uses DNA Nanoballs (DNBs) prepared from your library and loaded onto Patterned Array flow cells. Four fluorescent probes, each specific to one DNA base, are excited by lasers. The resulting high-resolution images are then analyzed by proprietary algorithms, delivering accurate sequencing results.

Circularization

DNB Making

DNB Loading

cPAS

2nd Strand Preparation

Base Calling Algorithm

Single-Strand DNA Circularization

Single-strand DNA circularization converts double-stranded DNA into a stable single-stranded circular template for accurate replication.

Double-stranded DNA is denatured into single strands with adapter sequences at the ends.

Circularization with a splint oligonucleotide joins the 5’ and 3’ ends, forming a nicked circle.

DNA ligase repairs the nick, creating a stable circular template.

How DNBSEQ™ Works

MGI’s DNBSEQ™ technology uses DNA Nanoballs (DNBs) prepared from your library and loaded onto Patterned Array flow cells. Four fluorescent probes, each specific to one DNA base, are excited by lasers. The resulting high-resolution images are then analyzed by proprietary algorithms, delivering accurate sequencing results.

Circularization

DNB Making

DNB Loading

cPAS

2nd Strand Preparation

Base Calling Algorithm

Single-Strand DNA Circularization

Single-strand DNA circularization converts double-stranded DNA into a stable single-stranded circular template for accurate replication.

Double-stranded DNA is denatured into single strands with adapter sequences at the ends.

Circularization with a splint oligonucleotide joins the 5’ and 3’ ends, forming a nicked circle.

DNA ligase repairs the nick, creating a stable circular template.

How DNBSEQ™ Works

MGI’s DNBSEQ™ technology uses DNA Nanoballs (DNBs) prepared from your library and loaded onto Patterned Array flow cells. Four fluorescent probes, each specific to one DNA base, are excited by lasers. The resulting high-resolution images are then analyzed by proprietary algorithms, delivering accurate sequencing results.

Circularization

DNB Making

DNB Loading

cPAS

2nd Strand Preparation

Base Calling Algorithm

Single-Strand DNA Circularization

Single-strand DNA circularization converts double-stranded DNA into a stable single-stranded circular template for accurate replication.

Double-stranded DNA is denatured into single strands with adapter sequences at the ends.

Circularization with a splint oligonucleotide joins the 5’ and 3’ ends, forming a nicked circle.

DNA ligase repairs the nick, creating a stable circular template.

Join our newsletter to stay up to date on features and releases.

I have read and understood MGI’s Privacy Policy, and I consent to the collection and processing of my personal data for handling, responding to my contact, receiving your newsletter as well as promotion and marketing activities.

*For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.

Join our newsletter to stay up to date on features and releases.

I have read and understood MGI’s Privacy Policy, and I consent to the collection and processing of my personal data for handling, responding to my contact, receiving your newsletter as well as promotion and marketing activities.

*For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.

Join our newsletter to stay up to date on features and releases.

I have read and understood MGI’s Privacy Policy, and I consent to the collection and processing of my personal data for handling, responding to my contact, receiving your newsletter as well as promotion and marketing activities.

*For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.