Comprehensive, sensitive and accurate HIV-1 genotyping and DRM detection
HIV drug resistance poses a significant global challenge to the sustainability of antiretroviral therapy and is the most common cause of antiviral treatment failure in HIV patients. Next Generation Sequencing holds significant promise in providing scalable throughput, increased sensitivity and accuracy for detecting drug resistance mutations.
MGI has developed an HIV-1 drug resistance sample to report workflow to accurately characterize HIV drug resistance in both plasma and culture specimens. Based on deep sequencing, capable of detecting drug resistance with low-frequency mutation, and combined with MGI’s automated
Sample Prep systems and HIV-GenomePro software, provides users with a streamlined tool for enabling rapid and accurate detection of HIV-1, drug resistance analysis, genome assembly, mutation detection, and evolutionary tracing.
MGI’s HIV -1 genotyping and drug resistance detection package can be deployed as a comprehensive and scalable solution, with the potential to improve individual patient care, as well as contributing to the ongoing research and genomic surveillance critical for the development of effective public health strategies in the fight against HIVDR and Antimicrobial Resistance.
HIV-1 Drug Resistance Sequencing workflow
Library Preparation
~7.5h
MGISP-960/MGISP-100
ATOPlex HIV-1 Amplifications Kit
MGIEasy Fast PCR-FREE FS DNA Library Prep Set
Sequencing
~12-40h
High-throughput Sequencing Set
DNBSEQ-G99 SM FCL PE150
DNBSEQ-E25 FCL PE150
Analysis
~1-2h
Precisely analyze and annotate drug resistance and explore with HIV-GenomePro Software
Features
Genotype/subtype annotation
Data quality control
Coverage statistics
PR-RT-IN gene assembly
Virus evolution tracing analysis
SNP and InDel detection
PR-RT-IN gene drug resistance annotation based on Stanford University HIVDB database
